人血小板堿性蛋白(PBP)ELISA試劑盒
實(shí)驗(yàn)原理：
本試劑盒用于測(cè)定人血清，血漿及相關(guān)液體樣本中血小板堿性蛋白(PBP)的含量。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中 人血小板堿性蛋白(PBP)水平。用純化的人血小板堿性蛋白(PBP)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中加入血小板堿性蛋白(PBP)，再與HRP標(biāo)記的血小板堿性蛋白(PBP)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的血小板堿性蛋白(PBP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中人血小板堿性蛋白(PBP)的濃度。
Human plaet basic protein(PBP)ELISA Kit
Principle of the assay
This kit allows for the determination of PBP concentrations in Human serum, blood plasma, and other biological fluids.The kit assay Human PBP level in the sample，use Purified Human PBP to coat microtiter plate wells, make solid-phase antibody, then add PBP to wells, Combined PBP antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA試劑盒供應(yīng)商：上海華壹生物科技有限公司
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