人鈣蛋白酶(calpain)ELISA試劑盒實驗事項：
1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。
2． 濃洗滌液可能會有結(jié)晶析出，稀釋時可在水浴中加溫助溶，洗滌時不影響結(jié)果。
3． 各步加樣均應(yīng)使用加樣器，并經(jīng)常校對其準(zhǔn)確性，以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。
4． 請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值），請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定，計算時請zui后乘以總稀釋倍數(shù)（×n×5）。
5． 封板膜只限一次性使用，以避免交叉污染。
6． 底物請避光保存，本試劑不同批號組分不得混用。
7． 嚴(yán)格按照說明書的操作進行，試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8． 所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。
兔ELISA試劑盒供應(yīng)商：上海華壹生物科技有限公司
Human calpain ELISA Kit
Important notes
- The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
- washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
- add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
- if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
- Closure plate membrane only limits the disposable use, to avoid cross-contamination.
- The substrate evade the light preservation.
- Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
- All samples, washing buffer and each kind of reject should according to infective material process.
- Do not mix reagents with those from other lots.
